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Image Search Results
Journal: PLoS ONE
Article Title: Tg2576 Cortical Neurons That Express Human Ab Are Susceptible to Extracellular Aβ-Induced, K + Efflux Dependent Neurodegeneration
doi: 10.1371/journal.pone.0019026
Figure Lengend Snippet: Tau immunolabelling of the axonal cytoskeleton demonstrated that axonal morphology was similar between wildtype (A) and Tg2576 (B) cortical neurons over 7–14 DIV. Six daily 1 µM Aβ 1-40 treatments of 7DIV wildtype neurons had no discernible effect upon axonal morphology (C). However, substantial changes in tau-labelling were observed in Aβ 1-40 treated Tg2576 neurons (D); including increased intensity of tau immunostaining after 24 hours, followed by blebbing and axonal fragmentation which worsened after four days of treatment (D). Furthermore, after four consecutive days of 1 µM Aβ 1-40 treatment a number of axonal swellings, with dense accumulations of hyperphosphorylated tau, were observed in Tg2576 neuron cultures (E). scale bars = 30 µm (A–D), 15 µm (E).
Article Snippet: For immunocytochemistry, rabbit anti-tau (1∶5000; DAKO),
Techniques: Immunostaining
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience
Article Title: Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines
doi: 10.1523/JNEUROSCI.6194-09.2010
Figure Lengend Snippet: (A) Representative samples from the canine parietal cortex show the anti-oligomer A11 antibody detecting the Aβ oligomeric protein at 56kDa. (B) Lower levels and reduced variability of the 56kDa aggregate are seen in the combination treatment group EA despite a large amount of variability within the groups. (CC=control environment/control diet, EC=behavioral enrichment/control diet, CA=control environment/antioxidant diet, EA=behavioral enrichment/antioxidant diet, IP=immunoprecipitated canine sample with anti-Aβ1-16 6E10 antibody, numbers on left in A=kilodalton marker, kDa=kilodalton, OD=optical density; GAPDH=glyceraldehyde 3-phosphate dehydrogenase)
Article Snippet: The antibodies used in Western blots were β-actin (rabbit, 1:5000, Abcam Inc., Cambridge, MA), GAPDH (rabbit, 1:10,000; Chemicon, Temecula, CA), the N-terminal
Techniques: Immunoprecipitation, Marker